Haematological and Molecular Characterisation of Haemoglobin E with Deletional Alpha-Thalassaemia among Kelantan Subjects

Heterozygous haemoglobin E (Hb E) with deletional alpha (α)-thalassaemia is commonly seen in Southeast Asia including Kelantan, a Northeastern state of Malaysia. Studies Malaysia showed that Hb the commonest among Malay Penang and Senoi group Orang Asli [1-5].
 
 This study aimed to compare haematological parameters (Hb, RBC, MCV, MCH, RDW heterozygous α-thalassaemia subjects. also intended ascertain cut-off value for using automated capillary electrophoresis method. A cross-sectional was conducted involving secondary data collection 219 samples possible from all districts Kelantan. Full blood count (FBC) analysed Sysmex XN 3000 cell analyser (Kobe, Japan). Hemoglobin (Hb) analysis performed CE system (Capillarys2; Sebia, France). Molecular characterisation multiplex gap-PCR ARMS-PCR detect both non-deletional α-thalassaemia, respectively. Qualitative were expressed as frequency percentage while quantitative mean, ± SEM median. One-way ANOVA used test mean level, RBC count, MCV MCH level differences six groups concurrent thalassaemia this study. The ROC curve determine point level.
 Eighty-nine confirmed have α-thalassaemia. α3.7 gene deletion most common observed 56 (62.9%) followed by SEA which detected 21 (23.6%). there significant difference between groups. predicting method 24.7% (Table 1). diagnostic performance sensitivity, specificity, positive predictive (PPV) negative (NPV) 100% all. can be assisting selection individual proceed DNA